Proximal regions of the olfactory marker protein gene promoter direct olfactory neuron-specific expression in transgenic mice

The human low affinity receptors for the Fc domain of immunoglobulin G, Fc gamma RIII, are encoded by two genes (IIIA and IIIB) which share >95% sequence identity in both coding and flanking sequences. Despite this extraordinary sequence conservation, IIIA is expressed in natural killer (NK) cells and macrophages and is absent in neutrophils, whereas IIIB is expressed only in neutrophils. To determine the molecular basis for this differential expression, we have generated transgenic mice using the genomic sequences of IIIA and IIIB. IIIA and IIIB transgenic mice show faithful reconstitution of this human pattern of cell type specificity. To determine the cis acting sequence elements that confer this specificity, we constructed chimeric genes in which 5.8 kb of 5' sequences of the IIIB gene has been replaced with a homologous region from the IIIA gene, and conversely, IIIA 5' sequences have been substituted for the analogous region of the IIIB gene. Promoter swap transgenic mice that carry IIIA 5' flanking sequences express Fc gamma RIII in macrophages and NK cells. In contrast, promoter swap transgenic mice that contain IIIB 5' sequences express Fc gamma RIII in neutrophils only. These studies define the elements conferring the cell type-specific expression of the human Fc gamma RIII genes within the 5' flanking sequences and first intron of the human Fc gamma RIIIA and Fc gamma RIIIB genes.

Download Full-text

Ha-ras oncogene expression directed by a milk protein gene promoter: tissue specificity, hormonal regulation, and tumor induction in transgenic mice.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.84.5.1299 ◽

1987 ◽

Vol 84 (5) ◽

pp. 1299-1303 ◽

Cited By ~ 117

Author(s):

A. C. Andres ◽

C. A. Schonenberger ◽

B. Groner ◽

L. Hennighausen ◽

M. LeMeur ◽

...

Keyword(s):

Transgenic Mice ◽

Hormonal Regulation ◽

Milk Protein ◽

Tissue Specificity ◽

Protein Gene ◽

Gene Promoter ◽

Ras Oncogene ◽

Tumor Induction ◽

Milk Protein Gene ◽

Oncogene Expression

Download Full-text

Ksp-cadherin gene promoter. II. Kidney-specific activity in transgenic mice

AJP Renal Physiology ◽

10.1152/ajprenal.1999.277.4.f599 ◽

1999 ◽

Vol 277 (4) ◽

pp. F599-F610 ◽

Cited By ~ 22

Author(s):

Peter Igarashi ◽

Cooduvalli S. Shashikant ◽

R. Brent Thomson ◽

Dilys A. Whyte ◽

Shuxian Liu-Chen ◽

...

Keyword(s):

Epithelial Cells ◽

Transgenic Mice ◽

Collecting Duct ◽

Gene Promoter ◽

Regulatory Elements ◽

Developmental Expression ◽

Tubular Epithelial Cells ◽

Specific Expression ◽

Tissue Specific

Kidney-specific cadherin (Ksp-cadherin, cadherin 16) is a tissue-specific member of the cadherin superfamily that is expressed exclusively in the basolateral membrane of tubular epithelial cells in the kidney. To determine the basis for tissue-specific expression of Ksp-cadherin in vivo, we evaluated the activity of the promoter in transgenic mice. Transgenic mice containing 3.3 kb of the mouse Ksp-cadherin promoter and an Escherichia coli lacZ reporter gene were generated by pronuclear microinjection. Assays of β-galactosidase enzyme activity showed that the transgene was expressed exclusively in the kidney in both adult and developing mice. Within the kidney, the transgene was expressed in a subset of renal tubular epithelial cells that endogenously expressed Ksp-cadherin and that were identified as collecting ducts by colabeling with Dolichos biflorus agglutinin. In the developing metanephros, expression of the transgene in the branching ureteric bud correlated with the developmental expression of Ksp-cadherin. Identical patterns of expression were observed in multiple founder mice, indicating that kidney specificity was independent of transgene integration site. However, heterocellular expression was observed consistent with repeat-induced gene silencing. We conclude that the Ksp-cadherin gene promoter directs kidney-specific expression in vivo. Regulatory elements that are sufficient to recapitulate the tissue- and differentiation-specific expression of Ksp-cadherin in the renal collecting duct are located within 3.3 kb upstream to the transcriptional start site.

Download Full-text